Contents
viii
Chapter 5. Biochemical Reaction Networks
119
5.1 Basic Concepts
119
5.2 Growth Energetics
124
5.2.1 Consumption of ATP for Cellular Maintenance
125
5.2.2 Energetics of Anaerobic Processes
128
5.2.3 Energetics of Aerobic Processes
132
5.3 Simple Metabolic Networks
142
5.4 Flux Analysis in Large Metabolic Networks
151
5.4.1 Use of Measurable Rates
153
5.4.2 Use of Labeled Substrates
163
5.4.3 Use of Linear Programming
171
Problems
179
References
186
Chapter 6. Enzyme Kinetics and Metabolic Control Analysis
189
6.1 Michaelis-Menten and Analogous Enzyme Kinetics
190
6.2 More Complicated Enzyme Kinetics
195
6.2.1 Variants of Michaelis-Menten Kinetics
195
6.2.2 Cooperativity and Allosteric Enzymes
201
6.3 Metabolic Control Analysis
207
Problems
233
References
234
Chapter 7. Modeling of Growth Kinetics
235
7.1 Model Structure and Complexity
237
7.2 A General Structure for Kinetic Models
240
7.2.1 Specification of Reaction Stoichiometries
240
7.2.2 Reaction Rates
242
7.2.3 Dynamic Mass Balances
244
7.3 Unstructured Growth Kinetics
245
7.3.1 The Black Box Model
245
7.3.2 Multiple Reaction Models
253
7.3.3 The Influence of Temperature and pH
261
7.4 Simple Structured Models
265
7.4.1 Compartment Models
265
7.4.2 Cybernetic Models
274
7.5 Mechanistic Models
278
7.5.1 Genetically Structured Models
279
7.5.2 Single Cell Models
289
7.6 Morphologically Structured Models
290
7.6.1 Oscillating Yeast Cultures
295
7.6.2 Growth of Filamentous Microorganisms
300
Problems
306
References
311
Chapter 8. Population Balance Equations
315
Problems
335
References
338
Chapter 9. Design of Fermentation Processes
339
9.1
The Stirred Tank Bioreactor
340
9.1.1 Batch Operation
342
9.1.2 The Continuous Stirred Tank Reactor
352
9.1.3 Biomass Recirculation
359
9.1.4 The Stirred Tank with Substrate Extracted from a Gas Phase
364
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