increase dramatically for increasing 1/s, since it is likely that
is determined experimentally with
a given absolute standard deviation irrespective of the value of
(which can always be
determined with a fixed relative standard error).
Once the correct type of inhibition has been found all parameters in the rate expression should be
determined by non-linear Least-Squares regression.
parameters should be shown together with the estimate of the parameter vector and the standard
deviation of the parameters.
Figure 6.2 Lineweaver-Burk (double reciprocal plots) of the relation between the rate
and the substrate
In all four plots the intersection P with the ordinate axis is k'1
in eq (6.1) for inhibitor
concentration 5, = 0 and the apparent value of
according to (6.10), (6.14), and (6.16) respectively. |Q|
is the corresponding value of
in (6.1) for s, = 0, and of the apparent value of
for 5, greater than
zero. The slope of the lines is equal to
Competitive inhibition by S|,eq(6.10).
Non-competitive inhibition by j,, eq (6.14).
Un-competitive inhibition by s,, eq (6.16)
Substrate inhibition by (6.16). Values of
are obtained from the ordinate at
the abscissa at Q. Slope of the inclined asymptote is