Bioreaction Engineering Principles
Jens Nielsen, John Villadsen, and Gunnar Liden
Biotechnology is a rapidly m oving field, which builds on the competence
and interplay of m any different disciplines — biochemistry, m icrobiology,
m olecular biology, and chemical engineering.The quantitative treatm ent of
biological processes today is a prerequisite for both the design of new
bioprocesses and the analysis of cellular function.
The present text is an extensively revised edition of the textbook first pub-
lished in 1994.The quantitative treatm ent of bioprocesses is a central them e
in this book.The book has been restructured to make it m ore easily accessi-
ble to the reader, the material has been updated and several new topics have
been added to the text.
The focus is on the bioreactor and the processes that occur in the reactor,
i.e., the coupling between the reactions occurring in the cell and its environ-
ment. The m icrobial cellular m etabolism is the starting point in the treat-
ment. Tools for the quantitative analysis of cellular functions —m acroscopic
mass balancing, therm odynam ics of m icrobial processes, m etabolic net-
w ork analysis, and kinetic m odelling —are gradually introduced. After analy-
sis of the cellular reactor, the interaction between the cell and its environ-
m ent is treated in chapters concerning mass transfer and design of bio-
processes. Finally, the com plex subject of scale-up is presented.
The book combines, in a rather unique way, a quantitative treatm ent of
physiology at the cellular level w ith a treatm ent of the bioreactor and inter-
action between the cellular reactor and the bioreactor. Many exam ples and
problem s are used to illustrate im portant concepts in the text.
Front cover illustration: Top: The inside of a stirred tank bioreactor. The
im peller shaft w ith tw o six-bladed Rushton turbines, baffles, and internal
cooling coils can be seen in the picture. Photo courtesy of Novozymes A/S,
Denmark. Bottom: Picture of the filam entous fungus
w ith DAPI staining of the nuclei. Photo courtesy of Center for Process
Biotechnology, DTU, Denmark.
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