From Cellular Function to Industrial Products
Besides application of these tools from the biosciences the design and proper operation of an
industrial plant for low-value added products requires a deep technical insight into many of the
disciplines that together makes up the science of chemical engineering: stoichiometric analysis,
modeling of reaction kinetics, transport phenomena and bioreactor design, and unit operations. In
this textbook we will highlight the chemical engineering disciplines that are associated with
bioreactions, we will give a quantitative description of a given phenotype of a cell, and will
illustrate how it operates in and interacts with technical equipment. A comprehensive treatment
of metabolic engineering is given in Stephanopoulos
et al.
(1998) or Nielsen (2001), while a
short (mostly provocative) account of the needs for engineering tools is given in Leib
et al.
Atkinson, D. E. (1977) Cellular Energy M etabolism and its Regulation. Academ ic Press, New York
Henriksen, C. M., Nielsen, J. & V illadsen, J. (1998). M odelling o f the protonophoric uncoupling by phenoxyacetic acid o f the
plasm a m em brane o f
P en icilliu m ch ryso g en u m . B io tech n o l. B io en g .
60:761 -767
Ingraham, J. L., M aaloe, O. & Neidhardt, F. C. (1983). G rowth o f the Bacterial Cell. Sinnauer Associates, Sunderland
Leib, T. M., Pereira, C. J. & Villadsen, J. (2001) Bioreactors: a chemical engineering perspective.
C hem . E ng. Sci.
Lengeler, J. W., Drews, G. & Schlegel, H. G. (1999) Biology o f the Prokaryotes. Thieme, Stuttgart
Neidhardt, F. C., Ingraham, J. L. & Schaechter, M. (1990). Physiology o f the Bacterial Cell. A
M olecular Approach. Sinauer
Associates, Sunderland
Nielsen, J. (1997). Physiological engineering aspects
o f P e n icilliu m ch ryso g en u m .
W orld Scientific Publishing Co., Singapore
Nielsen, J. (2001). M etabolic Engineering.
A p p l. M icrobiol. B io tech n o l.
Schulze, U. (1995). Anaerobic physiology o f
S a cc h a ro m yce s cerevisia e.
Ph.D. Thesis, Technical University o f Denmark
Sjoberg, A. & Hahn-Hagerdal, B. (1989) P-glucose-1-phosphate, a possible m ediator for polysaccharide formation in maltose-
L a cto co ccu s lactis. A ppl. E nviron. M icro b io l.
Stein, W. D. (1990). Channels, Carriers and Pumps. An Introduction to M em brane Transport. A cadem ic Press, San Diego
Stephanopoulos, G., Aristodou, A. & Nielsen, J. (1998). M etabolic Engineering. Principles and M ethodologies. Academic Press.
San Diego
Theobald, U., M ailinger, W. Reuss, M. & Rizzi, M. (1993).
In vivo
analysis o f glucose-induced
fast changes on yeast adenine
nucleotide pool applying a rapid sam pling technique.
A n a ly tic a l B io c h e m istiy
214, 31-37.
Theobald, U., M ailinger, W ., Baltes, M., Rizzi, M. & Reuss, M. (1997). In vivo analysis o f m etabolic dynam ics in
S a cch a ro m yces c e rev isia e
: I. Experim ental observations.
B io tech n o l. B io en g .
Vaseghi, S., Baum eister, A., Rizzi, M. & Reuss, M. (1999)
In vivo
dynam ics o f the pentose phosphate pathway in
S a cch a ro m yces cerevisiae. M etabol. E ng.
Verduyn, C , Postma, E., Scheffers, W. A. & van Dijken, J. P. (1992) Effect o f benzoic acid on metabolic tluxes in yeast. A
continuous culture study on the regulation o f respiration and alcoholic ferm entation.
Y east
8. 501-517
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